Enamours ethno medicinal properties with deficient in scientific evidence of a mangrove species Rhizophora mucronata distributed in Kollidam coast (Southeast coast of India). Therefore, we aimed to identify phyto constituents from alkaloid fraction of R.mucronata using GC-MS and evaluate their cytotoxic effect on HepG2 cells. 25g powdered leave material of R.mucronata used extract alkaloid fraction by acid-base extraction method and subjected to GC-MS analysis. The cytotoxic effect of alkaloid fraction on HepG2 cells at different concentration 20, 40, 60, 80 and l00 ?g/ml was confirmed by MTT assay. As a result 57 phyto-constituents identified from the alkaloids fraction. The major peaks showed eicosane, heptadecane, Diethyl phthalate, N-1-Adanantyl-p-methylbenzalimine and hepta decamine alkaloid derivatives present in significant concentration. The alkaloid fractions also possess cytotoxic effect against HepG2 cells. In conclusion R.mucronata derived alkaloids are potent chemotherapeutic agent at preclinical level.
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